Complement Factor D/Adipsin Antibody (255706)

• Reactivity: Hu
• Applications: WB
• Clone: 255706
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Factor D/Adipsin Antibody (255706) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Factor D
  Ile26-Ala253
  Accession # P00746
• Specificity: Detects human Complement Factor D in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFD
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor D/Adipsin Antibody (255706)

• Adipsin
• ADN
• ADNcomplement factor D
• AMBP-1
• C3 convertase activator
• CFD
• complement factor D (adipsin)
• complement factor D preproprotein
• Complement Factor D
• D component of complement (adipsin)
• DF
• EC 3.4.21
• EC 3.4.21.46
• PFD
• Properdin factor DADIPSIN

Background

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

1. White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
2. Taylor, F.R. et al. (1999) Biochemistry 38:2849.
3. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor D/Adipsin Antibody (MAB1824)(1)

Publication using MAB1824

• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010;56(0):1320. 2010 [PMID: 20530731] (Luminex Development, Human)

Bioinformatics

• Gene Symbol: CFD
• Uniprot:
  • P00746()