Recombinant Human Complement Component C5aR1 Protein

Product Discontinued

Recombinant Human Complement Component C5aR1 Protein Summary

• Description: Recombinant bioactive protein for Human Complement Component C5a R1
  
  

  Source:E. coli

• Details of Functionality: Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED50 = 1.36 nmolar.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: C5AR1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1.0 mg/ml in ice-cold H2O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70C for long-term storage. Frozen/thaw will result in some loss of activity.

Alternate Names for Recombinant Human Complement Component C5aR1 Protein

• C5a anaphylatoxin chemotactic receptor
• C5A
• C5aR
• C5a-R
• C5AR1
• C5ARC5a anaphylatoxin receptor
• C5R1C5a ligand
• CD88 antigen
• CD88
• complement component 5 receptor 1 (C5a ligand)
• complement component 5 receptor 1
• complement component 5a receptor 1
• Complement Component C5a R1
• Complement Component C5aR1

Background

Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the a-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli with a 6 amino acid, N-terminal His Tag sequence and 28 amino acid proprietary vector sequence necessary for expression. The recombinant protein is not further processed to remove the additional sequence. Recombinant C5a has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C5AR1