Recombinant Mouse Complement Component C5aR1 Protein

Product Discontinued

Recombinant Mouse Complement Component C5aR1 Protein Summary

• Description: Recombinant bioactive protein for Mouse Complement Component C5a R1
  
  

  Source:E. coli
  Amino Acid Sequence:(NP_034536)

• Details of Functionality: ED50 1-3 nM as determined by enzyme release assay of myeloperoxidase
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: C5AR1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-PAGE
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized with no additives
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold H2O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70C.

Alternate Names for Recombinant Mouse Complement Component C5aR1 Protein

• C5a anaphylatoxin chemotactic receptor
• C5A
• C5aR
• C5a-R
• C5AR1
• C5ARC5a anaphylatoxin receptor
• C5R1C5a ligand
• CD88 antigen
• CD88
• complement component 5 receptor 1 (C5a ligand)
• complement component 5 receptor 1
• complement component 5a receptor 1
• Complement Component C5a R1
• Complement Component C5aR1

Background

Mouse complement C5a is a 77-amino acid peptide generated during complement activation from thealpha-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C5AR1