Complement Component C5/C5a Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Complement Component C5/C5a Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Complement Component C5a (R&D Systems, Catalog # 2037-C5)
  Thr678-Arg751
  Accession # P01031
• Specificity: Detects human Complement Component C5/C5a in Western blots. In this format, approximately 5% cross‑reactivity with recombinant mouse Complement Component C5a is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: C5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5/C5a Antibody [Biotin]

• C5
• C5a;C5b;C5D;Complement C5;CPAMD4;ECLZB
• Complement Component C5/C5a

Background

Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C5/C5a Antibody (BAF2037)(1)

Publication using BAF2037

• CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare Kidney international reports, 2021-06-09;6(8):2105-2113. 2021-06-09 [PMID: 34386659] (Western Blot, Human)

Bioinformatics

• Gene Symbol: C5
• Uniprot:
  • P01031()