Complement Component C5/C5a Antibody [Unconjugated]

Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N‑acetyl‑ beta ‑D‑glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosamin­idase Release elicited by Recombinant Human Complement Component C5a (15 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Human Complement Component C5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2037). The ND50 is typically 0.15-0.75μg/mL in the presence of cytochalasin‑B.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Component C5/C5a Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Complement Component C5a
  Thr678-Arg751
  Accession # P01031
• Specificity: Detects human Complement Component C5/C5a in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse Complement Component C5a is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: C5
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5/C5a Antibody [Unconjugated]

• C5
• C5a;C5b;C5D;Complement C5;CPAMD4;ECLZB
• Complement Component C5/C5a

Background

Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C5/C5a Antibody (AF2037)(3)

Publications using AF2037

• Sexual dimorphism of liver metastasis by murine pancreatic neuroendocrine tumors is affected by expression of complement C5 Oncotarget, 2016-05-24;7(21):30585-96. 2016-05-24 [PMID: 27105526] (Western Blot, Human)
• Michael G. Strainic, Ethan M. Shevach, Fengqi An, Feng Lin, M. Edward Medof Absent C3a and C5a receptor signaling into CD4+ T cells enables auto-inductive TGF-beta 1 signaling and induction of Foxp3+ T regulatory cells Nature Immunology 2/1/2013 [PMID: 23263555]
• Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H Production of C5a by ASP, a serine protease released from Aeromonas sobria. J. Immunol., 2008-09-01;181(5):3602-8. 2008-09-01 [PMID: 18714034] (Western Blot, Human)

Bioinformatics

• Gene Symbol: C5
• Uniprot:
  • P01031()