Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

Immunohistochemistry: Complement Component C1s Antibody (C1s9) - (Pro and Active) - BSA Free [NBP1-05023] - Plasma shows distinct positivity stained with Complement Component C1s Antibody.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free Summary

• Immunogen: C1s proenzyme purified from human plasma
• Specificity: Specific for C1s proenzyme and activated free C1s
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C1S
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunohistochemistry 1:10-1:500
• Application Notes: ELISA: NBP1-05023 reacts in ELISA when tested with activated C1s enzyme directly coated onto the microtiter well or in sandwich ELISA in combination with a polyclonal antibody in coat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Component C1s Antibody (9) - (Pro and Active) - BSA Free

• basic proline-rich peptide IB-1
• C1 esterase
• C1S
• complement C1s subcomponent
• Complement component 1 subcomponent s
• complement component 1, s subcomponent
• Complement Component C1s
• EC 3.4.21
• EC 3.4.21.42
• FLJ44757

Background

C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes (1) but also by other cells (2,3). The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2 (1,4,5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C1S