Human Complement C8A ELISA Kit (Colorimetric)

ELISA: Human Complement C8A ELISA Kit (Colorimetric) [NBP2-60470]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Saliva, Milk, CSF
• Standard Curve Range: 0.625-40 ng/ml (example only; lot dependent)
• Sensitivity: 0.4 ng/ml (example only; lot dependent)

Human Complement C8A ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.625-40 ng/ml (example only; lot dependent)
• Sensitivity: 0.4 ng/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.20% (example only; lot dependent)
• Intra-Assay: CV% < 4.70% (example only; lot dependent)
• Spike Recovery: 103.00% (example only; lot dependent)
• Sample Volume: 50 ul (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C8A

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Complement C8A ELISA Kit (Colorimetric)

• complement component 8, alpha polypeptide
• complement component C8 alpha chain

Background

C8 is synthesized in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction. C8 is synthesized in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: C8A