Complement C5 Antibody (568)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IA
Clone
568
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Complement C5 Antibody (568) Summary

Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Specificity
This reacts with an epitope on C5 and on C5b.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
C5
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoassay
Application Notes
WB: 188.3 kDa. Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for Complement C5 Antibody (568)

  • anaphylatoxin C5a analog
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
  • C5
  • complement C5
  • complement component 5
  • CPAMD4MGC142298
  • FLJ17816
  • FLJ17822

Background

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Complement C5 Antibody (NB120-11879) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Complement C5 Antibody (NB120-11879). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in purchasing the following antibody: catalog # NB120-11879 Complement C5 Ab. In which epitope its been raised against? What volume do you get out of 0.05mg? Also, what is its cost and does it work on ELISA?
    • NB120-11879 is not epitope mapped but it is known that it reacts with an epitope on C5 and on C5b. The concentration is 0.1 mg/mL which is a volume of 500 uL and it has not yet been validated for use in ELISA. Regarding the price of this product, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information.

Secondary Antibodies

 

Isotype Controls

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Blogs on Complement C5.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

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Bioinformatics

Gene Symbol C5
Entrez