Human Complement C4b ELISA Kit (Colorimetric)

Human Complement C4b ELISA Kit (Colorimetric) - Standard Curve Reference

Product Discontinued

Human Complement C4b ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human C4b in samples. No significant cross-reactivity or interference between Human C4b and analogues was observed.
• Standard Curve Range: 4.69 - 300 ng/mL
• Sensitivity: 2.81 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.09%
• Intra-Assay: CV% < 4.87%
• Spike Recovery: 90-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C4B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Complement C4b ELISA Kit (Colorimetric)

• basic C4
• Basic complement C4
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
• C4B1
• C4B12
• C4B2
• C4B3
• C4FMGC164979
• CH
• Chido form of C4
• CO4C4B5
• complement C4-B
• complement C4B1a
• complement component 4B (Chido blood group)
• complement component 4B
• CPAMD3FLJ60561
• EC 2.1.1.144
• EC 2.7.11

Background

C4b encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Complement C4b ELISA Kit (NBP2-70046). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: C4B