Complement C4b/d Antibody (C4D204) [Alexa Fluor® 405]


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Product Details

Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Alexa Fluor 405

Order Details

Complement C4b/d Antibody (C4D204) [Alexa Fluor® 405] Summary

Recombinant human Complement 4d protein
Intracytoplasmic vacuoles of endothelial cells and Secreted
Acute Humoral Rejection Marker
This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
IgG1 Kappa
Protein A purified
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  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Complement C4b/d Antibody (C4D204) [Alexa Fluor® 405]

  • basic C4
  • Basic complement C4
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
  • C4B1
  • C4B12
  • C4B2
  • C4B3
  • C4FMGC164979
  • CH
  • Chido form of C4
  • CO4C4B5
  • complement C4-B
  • complement C4B1a
  • complement component 4B (Chido blood group)
  • complement component 4B
  • CPAMD3FLJ60561
  • EC
  • EC 2.7.11


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34581AF350
Alexa Fluor 405 NBP2-34581AF405
Alexa Fluor 488 NBP2-34581AF488
Alexa Fluor 532 NBP2-34581AF532
Alexa Fluor 594 NBP2-34581AF594
Alexa Fluor 647 NBP2-34581AF647
Alexa Fluor 700 NBP2-34581AF700
Alexa Fluor 750 NBP2-34581AF750
Biotin NBP2-34581B
DyLight 350 NBP2-34581UV
DyLight 405 NBP2-34581V
DyLight 488 NBP2-34581G
DyLight 550 NBP2-34581R
DyLight 594 NBP2-34581DL594
DyLight 650 NBP2-34581C
DyLight 680 NBP2-34581FR
DyLight 755 NBP2-34581IR
FITC NBP2-34581F
HRP NBP2-34581H
Janelia Fluor 549 NBP2-34581JF549
Janelia Fluor 646 NBP2-34581JF646

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Gene Symbol C4B