Complement C4b Antibody

Immunohistochemistry-Paraffin: Complement C4b Antibody [NBP1-50322] - Analysis of anti-C4B antibody with human placenta at concentration 20 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC-P
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Complement C4b Antibody Summary

• Immunogen: Purified human complement C4b.
• Specificity: Anti-Human Complement Component C4b polyclonal antibody reacts with human C4b. C4 is a 210 KDa trimeric glycoprotein containing three polypeptide chains alpha, beta, and gamma with respective molecular weights of 93KDa, 75KDa and 33 KDa. C4 breaks down in.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: C4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Paraffin 20 ug/ml

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Complement C4b Antibody

• basic C4
• Basic complement C4
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
• C4B1
• C4B12
• C4B2
• C4B3
• C4FMGC164979
• CH
• Chido form of C4
• CO4C4B5
• complement C4-B
• complement C4B1a
• complement component 4B (Chido blood group)
• complement component 4B
• CPAMD3FLJ60561
• EC 2.1.1.144
• EC 2.7.11

Background

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C4B