Complement C4b Antibody (25.3F4)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
25.3F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Complement C4b Antibody (25.3F4) Summary

Immunogen
C4 isolated from human plasma adsorbed onto aluminum hydroxide gel.
Specificity
Epitope specificity differs from that of NBP1-05084, as determined by inhibition ELISA.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
C4B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Theoretical MW
204 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Complement C4b Antibody (25.3F4)

  • basic C4
  • Basic complement C4
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
  • C4B1
  • C4B12
  • C4B2
  • C4B3
  • C4FMGC164979
  • CH
  • Chido form of C4
  • CO4C4B5
  • complement C4-B
  • complement C4B1a
  • complement component 4B (Chido blood group)
  • complement component 4B
  • CPAMD3FLJ60561
  • EC 2.1.1.144
  • EC 2.7.11

Background

C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b.Together C2a and C4b forms the classical C3 convertase. The molecular mass of C4 is 204 kDa and it consists of 3 domains: alpha (97kDa), beta (75 kDa) and gamma (33 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol C4B