ASP/C3a desArg Antibody (2991)

Immunoassay: ASP/C3a desArg Antibody (2991) [NB600-1210] - Three different batches tested in a sandwich ELISAto determine the specificity of clone 2991 (used as detection antibody)

• Reactivity: Hu
• Applications: WB, ELISA, ELISA
• Clone: 2991
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

ASP/C3a desArg Antibody (2991) Summary

• Immunogen: Human C3a des-Arg
• Specificity: This reacts with a neo-epitope (des-Arg) on C3a that is formed when C3 is cleaved into C3a and C3b.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Sandwich ELISA Detection
  • Western Blot 1:50-1:2000
• Application Notes: WB: A reduced sample treatment and SDS-PAGE was used. The band size is ~8 kDa .

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS containing 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for ASP/C3a desArg Antibody (2991)

• Acylation Stimulating Protein
• ASP
• C3a des Arg

Background

Acylation-stimulating protein (ASP), also known as C3adesArg, is an approximately 14 kDa member of the complement family that plays a role in the regulation of triglyceride synthesis (1-4). The secreted protein is a polypeptide fragment comprising amino acids (aa) 672-747 of the Complement C3 precursor protein (4). The polypeptide results from desargination of the carboxyl terminus of C3a, which is generated from interactions among C3, factor B, and adipsin (4). ASP contains a 36 aa anaphylatoxin-like domain (5). Human ASP shares 68% sequence identity with mouse and rat ASP. Increased levels of ASP are usually associated with insulin resistance, obesity and diabetes. It promotes triacylglycerol synthesis in adipocytes and inflammatory cytokine production in a septic environment (6-8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ASP/C3a desArg Antibody (NB600-1210) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Vishal Patel ELISA Human 08/13/2022

Summary

• Application: ELISA
• Sample Tested: Serum
• Species: Human
• Lot: 33459MO422

Bioinformatics

• Gene Symbol: C3
• Entrez:
  • 718(Human)
• Uniprot:
  • P01024()