Common gamma Chain/IL-2 R gamma Antibody

Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Common gamma Chain/IL‑2 R gamma Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF784). The ND50 is typically 6‑24 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Common gamma Chain/IL-2 R gamma Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Common gamma Chain/IL‑2 R gamma
  Ser25-Ala263
  Accession # Q3UPA9
• Specificity: Detects mouse Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-2 R gamma is observed and less than 1% cross-reactivity with rhIL-2 R alpha , recombinant mouse IL-2 R beta , and rhIL-15 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il2rg
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 6-24 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody

• CD132 antigen
• CD132
• CIDX
• combined immunodeficiency, X-linked
• common cytokine receptor gamma chain
• Common gamma Chain
• common gamma-chain
• cytokine receptor common subunit gamma
• gamma(c)
• IL-2 R gamma
• IL-2 receptor subunit gamma
• IL2R gamma
• IL-2R subunit gamma
• IL2RG
• IL-2RG
• IMD4
• interleukin 2 receptor, gamma
• Interleukin-2 receptor subunit gamma
• P64
• SCIDX
• SCIDX1
• severe combined immunodeficiency

Background

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma  is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Noguchi, M. et al. (1993) Science 262:1877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Common gamma Chain/IL-2 R gamma Antibody (AF784)(2)

Publications using AF784

• G Kim, Y Jo, B Lee, LA Ali, B Lee, C Hong The Potential Role of a Soluble ?-Chain Cytokine Receptor as a Regulator of IL-7-Induced Lymphoproliferative Disorders Int J Mol Sci, 2018-10-28;19(11):. 2018-10-28 [PMID: 30373315] (ELISA Development (Capture), Mouse)
• Lai L, Zeff RA, Goldschneider I A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells. Blood, 2005-11-22;107(5):1776-84. 2005-11-22 [PMID: 16304052] (Neutralization, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Il2rg
• Uniprot:
  • Q3UPA9()