Common beta Chain Antibody

Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Common beta Chain Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Common beta Chain
  Gly23-Trp443
  Accession # P32927
• Specificity: Detects recombinant human (rh) Common beta Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GM-CSF R alpha , rhG-CSF R and rhM-CSF R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSF2RB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Common beta Chain Antibody

• beta, low-affinity
• CD131 antigen
• CD131
• CDw131
• colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage)
• Common beta Chain
• CSF2RB
• cytokine receptor common subunit beta
• GM-CSF R beta
• GM-CSF/IL-3/IL-5 receptor common beta subunit
• GM-CSF/IL-3/IL-5 receptor common beta-chain
• IL-3 R beta
• IL3RB
• IL-5 R beta
• interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

Background

The common beta chain, also known as CD131, is a transmembrane protein that associates with the ligand-specific IL-3 R alpha , IL-5 R alpha , or GM-CSF R alpha proteins to form high affinity receptor complexes. beta c is expressed on a variety of hematopoietic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Common beta Chain Antibody (AF906)(2)

Publications using AF906

• Lei JT, Mazumdar T, Martinez-Moczygemba M Three lysine residues in the common beta chain of the interleukin-5 receptor are required for Janus kinase (JAK)-dependent receptor ubiquitination, endocytosis, and signaling. J. Biol. Chem., 2011-09-30;286(46):40091-103. 2011-09-30 [PMID: 21965659] (Western Blot, Human)
• Lei JT, Martinez-Moczygemba M Separate endocytic pathways regulate IL-5 receptor internalization and signaling. J. Leukoc. Biol., 2008-05-29;84(2):499-509. 2008-05-29 [PMID: 18511572] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CSF2RB
• Uniprot:
  • P32927()