GM-CSFR alpha Antibody (698423) [Unconjugated]

Images

 
GM‑CSF R alpha was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse GM‑CSF R alpha Mono­clonal Antibody (Catalog # MAB6130) at 10 µg/mL for 3 hours at ...read more
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM‑CSF R alpha Monoclonal Antibody (Catalog # MAB6130, filled histo­gram) or isotype control antibody (Catalog # MAB006, ...read more
Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.1 ng/mL) is ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clone
698423
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

GM-CSFR alpha Antibody (698423) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha
Leu30-Pro327
Accession # Q00941
Specificity
Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or
rhGM-CSF R beta is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CSF2RA
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.02-0.12 ug/mL
Publications
Read Publications using
MAB6130 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSFR alpha Antibody (698423) [Unconjugated]

  • CD116 antigen
  • CD116
  • CDw116
  • colony stimulating factor 2 receptor alpha subunit
  • colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage)
  • CSF2R
  • CSF2RA
  • CSF2RAX
  • CSF2RAY
  • CSF2RX
  • CSF2RY
  • GM-CSF R alpha
  • GM-CSF receptor alpha subunit
  • GMCSFR alpha
  • GMCSFR
  • GM-CSFRa
  • GM-CSF-R-alpha
  • GMCSFR-alpha
  • GMR
  • GMR-alpha
  • granulocyte-macrophage colony-stimulating factor receptor alpha chain
  • granulocyte-macrophage colony-stimulating factor receptor subunit alpha
  • MGC3848
  • MGC4838

Background

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
  6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
  8. Chiba, S. et al. (1990) Cell Regul. 1:327.
  9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
  10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GM-CSFR alpha Antibody (MAB6130)(4)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6130U
Alexa Fluor 405 FAB6130V
Alexa Fluor 488 FAB6130G
Alexa Fluor 594 FAB6130T
Alexa Fluor 647 FAB6130R
Alexa Fluor 700 FAB6130N
Alexa Fluor 750 FAB6130S
Allophycocyanin FAB6130A
Phycoerythrin FAB6130P

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Bioinformatics

Gene Symbol CSF2RA
Uniprot