COL9A3 Overexpression Lysate

Images

 
Western Blot: COL9A3 Overexpression Lysate (Adult Normal) [NBL1-09360] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COL9A3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

COL9A3 Overexpression Lysate Summary

Description

COL9A3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204010

Accession#: NM_001853

Protein Tag: C-MYC/DDK
Gene
COL9A3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COL9A3 Overexpression Lysate

  • collagen type IX proteoglycan
  • collagen, type IX, alpha 3
  • DJ885L7.4.1
  • EDM3collagen alpha-3(IX) chain
  • FLJ90759
  • IDDcollagen IX, alpha-3 polypeptide
  • MED

Background

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
DCMP0
Species: Hu
Applications: ELISA
NBP1-77022
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-90044
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56149
Species: Hu
Applications: IHC, IHC-P, IP, WB
NBP2-30450
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-49271
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-99960
Species: Hu
Applications: ELISA, IHC, IHC-P, WB
210-TA
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
202-IL
Species: Hu
Applications: BA
MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
AF682
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
AF5708
Species: Hu
Applications: ICC, WB

Publications for COL9A3 Lysate (NBL1-09360) (0)

There are no publications for COL9A3 Lysate (NBL1-09360).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for COL9A3 Lysate (NBL1-09360) (0)

There are no reviews for COL9A3 Lysate (NBL1-09360). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for COL9A3 Lysate (NBL1-09360) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional COL9A3 Products

Bioinformatics Tool for COL9A3 Lysate (NBL1-09360)

Discover related pathways, diseases and genes to COL9A3 Lysate (NBL1-09360). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for COL9A3 Lysate (NBL1-09360)

Discover more about diseases related to COL9A3 Lysate (NBL1-09360).
 

Pathways for COL9A3 Lysate (NBL1-09360)

View related products by pathway.

PTMs for COL9A3 Lysate (NBL1-09360)

Learn more about PTMs related to COL9A3 Lysate (NBL1-09360).

Blogs on COL9A3

There are no specific blogs for COL9A3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our COL9A3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol COL9A3