Cofilin 2 Antibody

Western Blot: Cofilin 2 Antibody [NB600-800] - HEK293 overexpressing Human Muscle Cofilin 2 and probed with (mock transfection in first lane).

Western Blot: Cofilin 2 Antibody [NB600-800] - Analysis of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cofilin 2 Antibody Summary

• Description: Novus Biologicals Goat Cofilin 2 Antibody (NB600-800) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cofilin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DIKDRSTLGEK corresponding to C-Terminus according to NP_068733.1, NP_619579.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CFL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 18 kDa band observed in human duodenum, heart, and skeletal muscle lysates and in mouse/rat heart lysates (calculated MW of 18.7 kDa band according to NP_068733.1and NP_619579.1).
• Control:

  Cofilin 2 Overexpression Lysate
  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cofilin 2 Antibody

• cofilin 2 (muscle)
• Cofilin, muscle isoform
• cofilin-2
• NEM7

Background

This antibody is expected to recognize both reported isoforms (NP_068733 and NP_619579)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cofilin 2 Antibody (NB600-800)(1)

Publication using NB600-800

• Thirion C, Stucka R, Mendel B et al. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem 2001-06-01 [PMID: 11422377]

Bioinformatics

• Gene Symbol: CFL2
• Entrez:
  • 1073(Human)
• Uniprot:
  • NP_068733.1(Human)