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![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Cofilin 2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98910-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98910] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Cofilin 2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-98910-img0005.jpg)
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98910] - Immunochemical staining Cofilin 2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-98910-img0004.jpg)
![Immunoprecipitation: Cofilin 2 Antibody [NBP2-98910] -Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 µL anti-CFL2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-CFL2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-98910_rabbit-polyclonal-cofilin-2-antibody-immunoprecipitation-852024115611..jpg)
![Western Blot: Cofilin 2 Antibody [NBP2-98910] -Lane A: Raji Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 μg per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/antibody/nbp2-98910_rabbit-polyclonal-cofilin-2-antibody-western-blot-852024115711..jpg)
![Western Blot: Cofilin 2 Antibody [NBP2-38544] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Western-Blot-NBP2-38544-img0003.jpg "Western Blot: Cofilin 2 Antibody [NBP2-38544] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue")
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-38544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38544-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-38544] - Staining in human skeletal muscle and pancreas tissues using anti-CFL2 antibody. Corresponding CFL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38544-img0007.jpg)
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-38544] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38544-img0005.jpg)
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-38544] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-38544-img0006.jpg)
![Western Blot: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of CFL2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-6G9-Western-Blot-H00001073-M03-img0008.jpg "Western Blot: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of CFL2 expression in HeLa (Cat # L013V1).")
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-6G9-Immunocytochemistry-Immunofluorescence-H00001073-M03-img0006.jpg)
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 8 ug/ml](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-6G9-Immunohistochemistry-Paraffin-H00001073-M03-img0007.jpg)
![ELISA: Cofilin 2 Antibody (6G9) [H00001073-M03] - Detection limit for recombinant GST tagged CFL2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-6G9-ELISA-H00001073-M03-img0004.jpg)
![Western Blot: Cofilin 2 Antibody [NBP1-33570] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Western-Blot-NBP1-33570-img0009.jpg "Western Blot: Cofilin 2 Antibody [NBP1-33570] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP1-33570] - Paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33570-img0005.jpg)
![Western Blot: Cofilin 2 Antibody [NBP1-33570] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Western-Blot-NBP1-33570-img0006.jpg)
![Western Blot: Cofilin 2 Antibody [NBP1-33570] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (NBP1-33570) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-33570_rabbit-polyclonal-cofilin-2-antibody-910202420560.jpg)
![Western Blot: Cofilin 2 Antibody [NB600-800] - HEK293 overexpressing Human Muscle Cofilin 2 and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Western-Blot-NB600-800-img0002.jpg "Western Blot: Cofilin 2 Antibody [NB600-800] - HEK293 overexpressing Human Muscle Cofilin 2 and probed with (mock transfection in first lane).")
![Western Blot: Cofilin 2 Antibody [NB600-800] - Analysis of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Western-Blot-NB600-800-img0001.jpg)
![Western Blot: Cofilin 2 Antibody [NBP2-92683] - Western blot analysis of extracts of various cell lines, using Cofilin 2 antibody (NBP2-92683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cofilin 2 Antibody-Western Blot-NBP2-92683-img0002.jpg "Western Blot: Cofilin 2 Antibody [NBP2-92683] - Western blot analysis of extracts of various cell lines, using Cofilin 2 antibody (NBP2-92683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-92683] - Immunofluorescence analysis of A549 cells using Cofilin 2 antibody (NBP2-92683). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92683-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98909] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Cofilin 2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98909-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-98909] - Immunofluorescence staining of Cofilin 2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Cofilin 2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: Cofilin 2 Antibody [NBP2-98909] - Immunochemical staining of human Cofilin 2 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cofilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-98909-img0002.jpg)
![ELISA: Human Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75254] - Samples were spiked with high concentrations of Human Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75254_human-cofilin-2-elisa-kit-colorimetric-132202416235735.jpg "ELISA: Human Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75254] - Samples were spiked with high concentrations of Human Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75254] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75254_human-cofilin-2-elisa-kit-colorimetric-432024983583.jpg)
![ELISA: Mouse Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75256] - Samples were spiked with high concentrations of Mouse Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75256_mouse-cofilin-2-elisa-kit-colorimetric-1322024162616.jpg "ELISA: Mouse Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75256] - Samples were spiked with high concentrations of Mouse Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75256] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75256_mouse-cofilin-2-elisa-kit-colorimetric-432024973685.jpg)
![ELISA: Rat Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75257] - Samples were spiked with high concentrations of Rat Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75257_rat-cofilin-2-elisa-kit-colorimetric-13220241613013.jpg "ELISA: Rat Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75257] - Samples were spiked with high concentrations of Rat Cofilin 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Cofilin 2 ELISA Kit (Colorimetric) [NBP2-75257] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75257_rat-cofilin-2-elisa-kit-colorimetric-4320249124834.jpg)
![Western Blot: Cofilin 2 Overexpression Lysate (Adult Normal) [NBP2-11264] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cofilin 2.](http://images.novusbio.com/fullsize/Cofilin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11264-img0001.jpg "Western Blot: Cofilin 2 Overexpression Lysate (Adult Normal) [NBP2-11264] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cofilin 2.")
![Western Blot: Cofilin 2 Overexpression Lysate (Adult Normal) [NBL1-09127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cofilin 2.](http://images.novusbio.com/fullsize/Cofilin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09127-img0002.jpg "Western Blot: Cofilin 2 Overexpression Lysate (Adult Normal) [NBL1-09127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cofilin 2.")