Cofilin 2 Antibody (6G9) - Azide and BSA Free

Western Blot: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of CFL2 expression in HeLa (Cat # L013V1).

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: Cofilin 2 Antibody (6G9) [H00001073-M03] - Analysis of monoclonal antibody to CFL2 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 8 ug/ml

ELISA: Cofilin 2 Antibody (6G9) [H00001073-M03] - Detection limit for recombinant GST tagged CFL2 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cofilin 2 Antibody (6G9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CFL2 (NP_068733, 57 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL
• Specificity: CFL2 - cofilin 2 (muscle)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFL2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cofilin 2 Antibody (6G9) - Azide and BSA Free

• cofilin 2 (muscle)
• Cofilin, muscle isoform
• cofilin-2
• NEM7

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cofilin 2 Antibody (H00001073-M03)(1)

Publication using H00001073-M03

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: CFL2
• Entrez:
  • 1073(Human)
• OMIM:
  • 601443(Human)
• Uniprot:
  • NP_068733(Human)