Coagulation Factor VII Antibody [Unconjugated]

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Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Coagulation Factor VII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3305) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Coagulation Factor VII Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Coagulation Factor VII
Ala42-Leu446
Accession # P70375
Specificity
Detects mouse Coagulation Factor VII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Factor VII, rhFactor X, and rhFactor Xa is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
F7
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF3305 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor VII Antibody [Unconjugated]

  • coagulation factor VII (serum prothrombin conversion accelerator)
  • Coagulation Factor VII
  • EC 3.4.21
  • EC 3.4.21.21
  • Eptacog alfa
  • F7
  • FVII coagulation protein
  • proconvertin
  • Serum prothrombin conversion accelerator
  • SPCA

Background

Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The deduced amino acid sequence of mouse factor VII predicts a signal peptide (residues 1 to 24), propeptide (residues 25 to 41), and the mature chain that can be further processed into the light chain (residues 42 to 193) and the heavy chain (residues 194 to 446). The amino acid sequence of mouse Factor VII is 89%, 71%, 65% and 55% identical to that of rat, human/dog, chimpanzee and chicken.

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. (eds. ), Academic Press, San Diego, p. 1659.
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coagulation Factor VII Antibody (AF3305)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ELISA Development, Flow.


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Bioinformatics

Gene Symbol F7
Entrez
Uniprot