Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

Raw 264.7 cells treated with 1 µg/mL LPS overnight were stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3178, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, IP, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Coagulation Factor III/Tissue Factor Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
  Ala29-Glu251
  Accession # P20352
• Specificity: Detects mouse Coagulation Factor III in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: F3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody [Unconjugated]

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (AF3178)(17)

Publications using AF3178

• CD Holman, AP Sakers, RP Calhoun, L Cheng, EC Fein, C Jacobs, L Tsai, ED Rosen, P Seale Aging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogramming bioRxiv : the preprint server for biology, 2023-03-23;0(0):. 2023-03-23 [PMID: 36993336] (Flow Cytometry, Mouse)
• Rosell A, Moser B, Hisada Y et al. Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting Res Pract Thromb Haemost 7/6/2020 [PMID: 32864552]
• Saumya Bhagat, Indranil Biswas, Rehan Ahmed, Gausal A. Khan Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling Blood Cells, Molecules, and Diseases 2020-09-01 [PMID: 32559654]
• X Chen, Y Lao, J Yi, J Yang, S He, Y Chen SENP3 in monocytes/macrophages up-regulates tissue factor and mediates lipopolysaccharide-induced acute lung injury by enhancing JNK phosphorylation J. Cell. Mol. Med., 2020-03-31;0(0):. 2020-03-31 [PMID: 32237051] (Western Blot, Mouse)
• Desirée Edén, Grigorios Panagiotou, Dariush Mokhtari, Jan W. Eriksson, Mikael Åberg, Agneta Siegbahn Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent manner Upsala Journal of Medical Sciences 2019-08-14 [PMID: 31407948]
• Y Oe, T Fushima, E Sato, A Sekimoto, K Kisu, H Sato, J Sugawara, S Ito, N Takahashi Protease-activated receptor 2 protects against VEGF inhibitor-induced glomerular endothelial and podocyte injury Sci Rep, 2019-02-27;9(1):2986. 2019-02-27 [PMID: 30814628] (IHC-P, Mouse)
• Stefan Uderhardt, Jochen A. Ackermann, Tobias Fillep, Victoria J. Hammond, Johann Willeit, Peter Santer et al. Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease Journal of Experimental Medicine 2017-07-03 [PMID: 28566277]
• Yunmei Wang, Huiyun Gao, Can Shi, Paul W. Erhardt, Alexander Pavlovsky, Dmitry A. Soloviev et al. Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIb alpha Nature Communications 2017-05-30 [PMID: 28555620]
• Y Zhang, L Li, Y Zhao, H Han, Y Hu, D Liang, B Yu, J Kou The Myosin II Inhibitor, Blebbistatin, Ameliorates FeCl3-induced Arterial Thrombosis via the GSK3?-NF-?B Pathway Int. J. Biol. Sci., 2017-05-15;13(5):630-639. 2017-05-15 [PMID: 28539835] (IHC, Mouse)
• Shaobin Wang, Brandi Reeves, Erica M. Sparkenbaugh, Janice Russell, Zbigniew Soltys, Hua Zhang et al. Protective and detrimental effects of neuroectodermal cell–derived tissue factor in mouse models of stroke JCI Insight 2016-07-21 [PMID: 27489885]
• Jessica R. Hitchcock, Charlotte N. Cook, Saeeda Bobat, Ewan A. Ross, Adriana Flores-Langarica, Kate L. Lowe et al. Inflammation drives thrombosis after Salmonella infection via CLEC-2 on platelets Journal of Clinical Investigation 2015-11-16 [PMID: 26571395]
• Pierre-Emmanuel Rautou, Kohei Tatsumi, Silvio Antoniak, A. Phillip Owens, Erica Sparkenbaugh, Lori A. Holle et al. Hepatocyte tissue factor contributes to the hypercoagulable state in a mouse model of chronic liver injury Journal of Hepatology 2016-01-01 [PMID: 26325534]
• Desirée Edén, Agneta Siegbahn, Dariush Mokhtari Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated insulin secretion from human pancreatic islets Diabetologia 2015-11-01 [PMID: 26271343]
• Ciara M. Shaver, Brandon S. Grove, Nathan D. Putz, Jennifer K. Clune, William E. Lawson, Robert H. Carnahan et al. Regulation of Alveolar Procoagulant Activity and Permeability in Direct Acute Lung Injury by Lung Epithelial Tissue Factor American Journal of Respiratory Cell and Molecular Biology 2015-11-01 [PMID: 25884207] (Immunohistochemistry, Western Blot, ELISA Detection, Transgenic Mouse)
• QING ZHANG, XIUJUN LIU, CAIHONG LI, DONGSHENG LIAO, ZHIGANG OUYANG, JUNNIAN ZHENG et al. Tissue factor-targeted lidamycin inhibits growth and metastasis of colon carcinoma Oncology Letters 2013-09-01 [PMID: 24137414] (Flow Cytometry, Mouse)
• Sebag S, Bastarache J, Ware L Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells. J Biol Chem, 2013-01-29;288(11):7875-84. 2013-01-29 [PMID: 23362270] (Western Blot, Mouse)
• Li F, Wang CH, Wang JG, Thai T, Boysen G, Xu L, Turner AL, Wolberg AS, Mackman N, Maeda N, Takahashi N Elevated tissue factor expression contributes to exacerbated diabetic nephropathy in mice lacking eNOS fed a high fat diet. J. Thromb. Haemost., 2010-10-01;8(10):2122-32. 2010-10-01 [PMID: 20626618] (Neutralization, Mouse)

FAQs for Coagulation Factor III/Tissue Factor Antibody (AF3178). (Showing 1 - 1 of 1 FAQs).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Uniprot:
  • P20352()