Coagulation Factor III/Tissue Factor Antibody (355220)

• Reactivity: Mu
• Applications: WB
• Clone: 355220
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Coagulation Factor III/Tissue Factor Antibody (355220) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III
  Ala29-Glu251
  Accession # P20352
• Specificity: Detects mouse Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Rat
• Gene: F3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (355220)

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 - 28) and the mature chain (residues 29 - 294). As a type I membrane protein, it contains a transmembrane region (residues 252 - 274) and a cytoplasmic tail (residues 275 - 294) (3, 4). The purified recombinant mouse TF corresponds to the ectodomain (residues 29 - 251) and is potent in activating thermolysin-processed recombinant mouse Coagulation Factor VII (R&D Systems, Catalog # 3305‑SE) under the conditions described in the Activity Assay Protocol.

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
3. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
4. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (MAB3178)(1)

Publication using MAB3178

• Hui Zhang, Ling Zeng, Min Xie, Jiao Liu, Borong Zhou, Runliu Wu et al. TMEM173 Drives Lethal Coagulation in Sepsis Cell Host & Microbe 2020-04-01 [PMID: 32142632]

FAQs for Coagulation Factor III/Tissue Factor Antibody (MAB3178). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Uniprot:
  • P20352()