CNPase Antibody

Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.

Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CNPase Antibody Summary

• Immunogen: Full length human CNPase recombinant protein. [UniProt# P09543]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:1000 - 1:2000
• Application Notes: In western blot, double bands may be detected at 46 and 48kDa, corresponding to the two isotypes of the CNP protein.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CNPase Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CNPase Antibody

• 2', 3' cyclic nucleotide 3' phosphohydrolase
• 2'-3'-cyclic nucleotide 3' phosphodiesterase
• 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
• CNP1
• CNPase
• EC 3.1.4.37

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNP
• Uniprot:
  • P09543(Human)