Natriuretic Peptide Receptor C Antibody (OTI4C3)

Western Blot: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Natriuretic Peptide Receptor C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Natriuretic Peptide Receptor C.

Immunocytochemistry/Immunofluorescence: Natriuretic Peptide Receptor C Antibody (OTI4C3) [NBP2-02401] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Natriuretic Peptide Receptor C.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded thyroid tissue using anti-RC219453 mouse monoclonal antibody.

Flow Cytometry: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - HEK293T cells transfected with either pCMV6-ENTRY Natriuretic Peptide Receptor C.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Natriuretic Peptide Receptor C mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded bladder tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded colon tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded Kidney tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded lung tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded pancreas tissue using anti-RC219453 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Staining of paraffin-embedded prostate tissue using anti-RC219453 mouse monoclonal antibody.

Flow Cytometry: Natriuretic Peptide Receptor C Antibody (4C3) [NBP2-02401] - Analysis of Jurkat cells, using anti-Natriuretic Peptide Receptor C antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Natriuretic Peptide Receptor C Antibody (OTI4C3) Summary

• Immunogen: Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 59.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Natriuretic Peptide Receptor C Antibody (OTI4C3)

• ANP-C
• ANPR-C
• ANPRCFLJ14054
• Atrial natriuretic peptide clearance receptor
• atrial natriuretic peptide receptor 3
• Atrial natriuretic peptide receptor type C
• C5orf23
• chromosome 5 open reading frame 23
• GUCY2B
• lgj
• natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C)
• NPR3
• NPRC
• NPR-C
• stri

Background

The family of natriuretic peptides (see MIM 108780) elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Natriuretic Peptide Receptor C Antibody (NBP2-02401)(1)

Publication using NBP2-02401

• Sun M, Chen Z, Song Y Et al. PLXND1-mediated calcium dyshomeostasis impairs endocardial endothelial autophagy in atrial fibrillation Front Physiol 2022-08-26 [PMID: 36017337] (FLOW, Mouse)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: NPR3
• Entrez:
  • 4883(Human)