CNPase Antibody

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Western Blot: CNPase Antibody [NB110-79874] - Analysis of CNPase in 10ug of normal brain tissue lysate using CNPase antibody NB110-79874 at a dilution of 1:500

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CNPase Antibody Summary

Immunogen
Reacts with residues 68-84 of the 46-48 (doublet) kDa human, CNPase protein (74% sequence identity and 94% sequence similarity to mouse and rat).
Marker
Oligodendrocyte Marker
Specificity
CNPase - Oligodendrocyte Marker
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CNP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This antibody is useful in Western Blot, ELISA and Immunohistochemistry

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CNPase Lysate (NBL1-09322)

Reactivity Notes

Human. 94% sequence identity with mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CNPase Antibody

  • 2', 3' cyclic nucleotide 3' phosphohydrolase
  • 2'-3'-cyclic nucleotide 3' phosphodiesterase
  • 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
  • CNP1
  • CNPase
  • EC 3.1.4.37

Background

CNPase (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase) exists in two forms: 46kDa (CNP1) and 48kDa (CNP2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtual absent in other cell types. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA 1 familial breast cancer gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CNP
Entrez