![Western Blot: CNOT8 Antibody [NBP2-15930] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody (NBP2-15930) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15930_rabbit-polyclonal-cnot8-antibody-91020242041262.jpg "Western Blot: CNOT8 Antibody [NBP2-15930] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody (NBP2-15930) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Pm, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary. |
| Localization | Cytoplasm; Nucleus |
| Predicted Species | Mouse (99%), Rat (100%), Chicken (97%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CNOT8 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Simple_Western_17480.jpg)
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| Publication using NBP2-15930 | Applications | Species |
|---|---|---|
| Li CY, Liang Z, Hu Y et al. Cytidine-containing tails robustly enhance and prolong protein production of synthetic mRNA in cell and in vivo Molecular therapy. Nucleic acids 2022-12-13 [PMID: 36320322] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CNOT8 Antibody (NBP2-15930)Find related products by research area.
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![Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] - CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CNOT8 protein stained by CNOT8 antibody (NBP2-15930) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15930_rabbit-polyclonal-cnot8-antibody-91020242021952.jpg "Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] - CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CNOT8 protein stained by CNOT8 antibody (NBP2-15930) diluted at 1:200.Blue: Hoechst 33342 staining.")
![Flow Cytometry CCR4 Antibody (205410) [Unconjugated]](https://images.novusbio.com/images2/CCR4_MAB1567_Flow_Cytometry_20627.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")