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CNOT8 Antibody

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Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.
Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, RM, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CNOT8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Predicted Species
Mouse (99%), Rat (100%), Chicken (97%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CNOT8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%), Zebrafish (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CNOT8 Antibody

  • CAF1
  • CAF2
  • CALIFCAF1-like protein
  • CALIFp
  • CCR4-NOT transcription complex subunit 8
  • CCR4-NOT transcription complex, subunit 8
  • hCAF1
  • PGK promoter directed over production
  • POP2CCR4-associated factor 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: CNOT6 Antibody [NBP1-86564] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunocytochemistry/Immunofluorescence: CNOT6 Antibody [NBP1-86564] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Nocturnin Antibody
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Immunohistochemistry-Paraffin: Nocturnin Antibody [NBP2-37856] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunocytochemistry/Immunofluorescence: Nocturnin Antibody [NBP2-37856] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CCR4 Antibody
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MAB1567
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
(19 Publications)
Species: Hu
Applications: CyTOF-reported, Flow
CNOT7 Antibody
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H00029883-M01
Immunocytochemistry/Immunofluorescence: CNOT7 Antibody (2F6) [H00029883-M01] - Analysis of monoclonal antibody to CNOT7 on HeLa cell. Antibody concentration 40 ug/ml.
Sandwich ELISA: CNOT7 Antibody (2F6) [H00029883-M01] - Detection limit for recombinant GST tagged CNOT7 is 3 ng/ml as a capture antibody.
Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6 Analysis of CNOT7 expression in HeLa.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
CNOT1 Antibody
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Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human fallopian tube shows weak to moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human duodenum shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CHAF1A Antibody
(SS 1 1-13)
NB500-207
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
(10 Publications)
Species: Hu
Applications: ICC/IF, IP, WB
CHAF1B Antibody
(SS 53)
NB500-212
Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.
(8 Publications)
Species: Hu
Applications: ICC/IF, IP, KD, WB
TOB1 Antibody
AF5228
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
RBBP4/RbAp48
Antibody (11G10)
NB500-123
Immunoprecipitation: RBBP4/RbAp48 Antibody (11G10) [NB500-123] - Myc-tag antibody immunoprecipitation confirmed pulldown of NuRD component RbAp48 which is known to interact with ZNF827. * denotes IgG band at 55 kDa. Image submitted by a verified customer review.
Immunohistochemistry: RbAp48 Antibody (1D11) [NB500-123]
Immunocytochemistry/Immunofluorescence: RbAp48 Antibody (1D11) [NB500-123] - Immunofluorescence analysis of HeLa, using RbAp48 antibody at 1:100 dilution.
(5 Publications)
Species: Hu, Mu
Applications: B/N, ChIP, Func, ICC/IF, IHC, IHC-P, IP, WB
SSB Antibody
(OTI2C8)
NBP1-48002
Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.
Species: Ca, Hu, Pm, Pm
Applications: ICC/IF, IHC, IHC-P, IP, WB
BTG2 Antibody
(OTI4C10)
NBP2-46587
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
WIRE Antibody
NBP1-86856
Western Blot: WIRE Antibody [NBP1-86856] - Analysis using Anti-WIPF2 antibody NBP1-86856 (A) shows similar pattern to independent antibody NBP1-86858 (B).
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human cerebral cortex, colon, fallopian tube and liver using Anti-WIRE antibody NBP1-86856 (A) shows similar protein distribution across tissues to independent antibody NBP1-86858 (B).
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Analysis in human cerebral cortex and liver tissues using NBP1-86856 antibody. Corresponding WIRE RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CNOT2 Antibody
NBP2-56034
Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP2-56034] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP2-56034] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP2-56034] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
NBP2-52486
ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
BTG3 Antibody
NBP1-89098
Western Blot: BTG3 Antibody [NBP1-89098] - Analysis in control (vector only transfected HEK293T lysate) and BTG3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: BTG3 Antibody [NBP1-89098] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells along with extracellular material.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
BTG1 Antibody
(OTI7H6)
NBP2-46586
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(8 Reviews)
(46 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
PARN Antibody
(OTI4D12)
NBP2-46321
Immunohistochemistry: PARN Antibody (4D12) [NBP2-46321] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PARN Antibody (4D12) [NBP2-46321] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PARN Antibody (4D12) [NBP2-46321] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu, Mu, Rt
Applications: IHC, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
(37 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol CNOT8
Uniprot