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CNOT8 Antibody

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Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.
Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Pm, RM, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CNOT8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Predicted Species
Mouse (99%), Rat (100%), Chicken (97%), Xenopus (95%), Primate (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CNOT8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (95%), Zebrafish (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for CNOT8 Antibody

  • CAF1
  • CAF2
  • CALIFCAF1-like protein
  • CALIFp
  • CCR4-NOT transcription complex subunit 8
  • CCR4-NOT transcription complex, subunit 8
  • hCAF1
  • PGK promoter directed over production
  • POP2CCR4-associated factor 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: CNOT6 Antibody [NBP1-86564] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: CNOT6 Antibody [NBP1-86564] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Nocturnin Antibody
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Immunocytochemistry/Immunofluorescence: Nocturnin Antibody [NBP2-37856] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Nocturnin Antibody [NBP2-37856] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Species: Hu
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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR4 Monoclonal Antibody (Catalog # MAB1567) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6. Analysis of CNOT7 expression in Raw 264.7.
Immunocytochemistry/Immunofluorescence: CNOT7 Antibody (2F6) [H00029883-M01] - Analysis of monoclonal antibody to CNOT7 on HeLa cell. Antibody concentration 40 ug/ml.
Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6 Analysis of CNOT7 expression in HeLa.
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CNOT1 Antibody
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Western Blot: CNOT1 Antibody [NBP2-31892] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: CNOT1 Antibody [NBP2-31892] - Immunofluorescent staining of human cell line SiHa shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CNOT1 Antibody [NBP2-31892] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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CHAF1A Antibody
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Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
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CHAF1B Antibody
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Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.
(5 Publications)
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TOB1 Antibody
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune mouse IgG, C. Immunoprecipitation of RbAp48 protein by 2. 5 ug of RbAp48 antibody 10% SDS-PAGE gel.
Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Chromatin Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG, B. 5 ug of RbAp48 antibody The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
(1 Review)
(4 Publications)
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SSB Antibody
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Western Blot: SSB Antibody [NBP1-33549] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SSB Antibody [NBP1-33549] - Analysis of paraformaldehyde-fixed A431, using SSB (NBP1-33549) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SSB Antibody [NBP1-33549] - Paraffin-embedded mouse fore brain. SSB antibody dilution: 1:1000.
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BTG2 Antibody
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Western Blot: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG 1.
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Western Blot: WIRE Antibody [NBP1-86856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human skeletal muscle shows low expression as expected.
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CNOT2 Antibody
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Western Blot: CNOT2 Antibody [NBP2-56034] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/Immunofluorescence: CNOT2 Antibody [NBP2-56034] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: CNOT2 Antibody [NBP2-56034] - Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
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HIST1H4F
Overexpression Lysate
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Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
H4/n Antibody
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Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded esophageal cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
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Immunohistochemistry: BTG1 Antibody (7H6) [NBP2-46586] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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CD117/c-kit
Antibody [Unconjugate
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Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
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Species: Hu, Mu
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PARN Antibody
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Western Blot: PARN Antibody (4D12) [NBP2-46321] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARN.
Immunohistochemistry: PARN Antibody (4D12) [NBP2-46321] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: PARN Antibody (4D12) [NBP2-46321] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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CD4 Antibody
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Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
(7 Reviews)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol CNOT8
Uniprot