CNOT8 Antibody

Western Blot: CNOT8 Antibody [NBP2-15930] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: CNOT8 Antibody [NBP2-15930] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CNOT8 protein stained by CNOT8 antibody diluted at 1:200. Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm, RM, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CNOT8 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Mouse (99%), Rat (100%), Chicken (97%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNOT8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%), Zebrafish (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CNOT8 Antibody

• CAF1
• CAF2
• CALIFCAF1-like protein
• CALIFp
• CCR4-NOT transcription complex subunit 8
• CCR4-NOT transcription complex, subunit 8
• hCAF1
• PGK promoter directed over production
• POP2CCR4-associated factor 8

Background

Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CNOT8 Antibody (NBP2-15930)(1)

Publication using NBP2-15930

• Li CY, Liang Z, Hu Y et al. Cytidine-containing tails robustly enhance and prolong protein production of synthetic mRNA in cell and in vivo Molecular therapy. Nucleic acids 2022-12-13 [PMID: 36320322] (WB, Human)

Bioinformatics

• Gene Symbol: CNOT8
• Uniprot:
  • Q9UFF9(Human)