CNOT7 Antibody

Images

 
Western Blot: CNOT7 Antibody [NBP2-15929] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: CNOT7 Antibody [NBP2-15929] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

CNOT7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CNOT7. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CNOT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (95%), Xenopus tropicalis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.84 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CNOT7 Antibody

  • BTG1 binding factor 1
  • BTG1-binding factor 1
  • CAF-1
  • CAF1carbon catabolite repressor protein (CCR4)-associative factor 1
  • CCR4-associated factor 1
  • CCR4-NOT transcription complex subunit 7
  • CCR4-NOT transcription complex, subunit 7
  • hCAF-1

Background

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CNOT7 Antibody (NBP2-15929) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CNOT7
Uniprot