CNOT7 Antibody - BSA Free

Western Blot: CNOT7 Antibody [NBP2-15929] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: CNOT7 Antibody [NBP2-15929] - Human colon carcinoma, using CNOT7 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CNOT7 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CNOT7. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Rat (99%), Chicken (99%), Xenopus (95%), Zebrafish (94%), Rhesus Macaque (99%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNOT7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF-Assay dependent
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CNOT7 Antibody - BSA Free

• BTG1 binding factor 1
• BTG1-binding factor 1
• CAF-1
• CAF1carbon catabolite repressor protein (CCR4)-associative factor 1
• CCR4-associated factor 1
• CCR4-NOT transcription complex subunit 7
• CCR4-NOT transcription complex, subunit 7
• hCAF-1

Background

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CNOT7
• Uniprot:
  • Q9UIV1(Human)