CLOCK Antibody (HL1099) - Azide and BSA Free

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB
• Clone: HL1099
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CLOCK Antibody (HL1099) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CLOCK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CLOCK Antibody (HL1099) - Azide and BSA Free

• BHLHE8
• circadian locomoter output cycles protein kaput
• Class E basic helix-loop-helix protein 8
• clock (mouse) homolog
• clock homolog (mouse)
• CLOCK
• EC 2.3.1.48
• hCLOCK
• KAT13D
• KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLOCK