CLGN Antibody (9C8G10) - BSA Free

Western Blot: CLGN Antibody (9C8G10) [NBP2-37395] - Western blot analysis using CLGN mouse mAb against LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate.

Immunocytochemistry/Immunofluorescence: CLGN Antibody (9C8G10) [NBP2-37395] - Immunofluorescence analysis of HepG2 cells using CLGN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: CLGN Antibody (9C8G10) [NBP2-37395] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CLGN mouse mAb with DAB staining.

Western Blot: CLGN Antibody (9C8G10) [NBP2-37395] - Western blot analysis using CLGN mAb against human CLGN recombinant protein. (Expected MW is 43.5 kDa)

Western Blot: CLGN Antibody (9C8G10) [NBP2-37395] - Western blot analysis using CLGN mAb against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: CLGN Antibody (9C8G10) [NBP2-37395] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CLGN mouse mAb with DAB staining.

ELISA: CLGN Antibody (9C8G10) [NBP2-37395] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 9C8G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CLGN Antibody (9C8G10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLGN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:75
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CLGN Antibody (9C8G10) - BSA Free

• calmegin

Background

Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLGN