CLGN Antibody (6U8D7)

Western Blot: CLGN Antibody (6U8D7) [NBP3-15836] - Western blot analysis of extracts of various cell lines, using CLGN Rabbit mAb (NBP3-15836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry: CLGN Antibody (6U8D7) [NBP3-15836] - Immunofluorescence analysis of mouse testis cells using CLGN Rabbit mAb (NBP3-15836) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: CLGN Antibody (6U8D7) [NBP3-15836] - Western blot analysis of extracts of PC-3 cells, using CLGN Rabbit mAb (NBP3-15836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: CLGN Antibody (6U8D7) [NBP3-15836] - Immunohistochemistry of paraffin-embedded rat testis using CLGN Rabbit mAb (NBP3-15836) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CLGN Antibody (6U8D7) [NBP3-15836] - Immunohistochemistry of paraffin-embedded mouse testis using CLGN Rabbit mAb (NBP3-15836) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry: CLGN Antibody (6U8D7) [NBP3-15836] - Immunofluorescence analysis of rat testis cells using CLGN Rabbit mAb (NBP3-15836) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 6U8D7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CLGN Antibody (6U8D7) Summary

• Description: Novus Biologicals Rabbit CLGN Antibody (6U8D7) (NBP3-15836) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-350 of human CLGN (O14967). FGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CLGN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CLGN Antibody (6U8D7)

• calmegin

Background

Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLGN