Claudin-6 Antibody (9H8) - BSA Free

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651] - The Binding Activity of Human Claudin-6 with Claudin-6 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Claudin-6 at 10 ug/mL can bind Claudin-6 Antibody, the EC50 is 1.501-2.035 ng/mL.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] - Untransfected HEK293T cells (green line) and transfected Human CLDN6 HEK293T stable cells (red line) were stained with anti-CLDN6/9 recombinant antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected CHO-K1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

ELISA: Claudin-6 Antibody (9H8) [NBP3-26651] -The Binding Activity of Macaca fascicularis CLDN6 with Anti-CLDN6/9 Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN6 at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC50 is 4.186-6.635 ng/mL.The VLPs (CSB-MP3838) is negative control.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected MC38 cells (green line) and transfected Human CLDN9 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Flow Cytometry: Claudin-6 Antibody (9H8) [NBP3-26651] -Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6/9 antibody (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

• Reactivity: Hu, Pm-Cm
• Applications: ELISA, Flow
• Clone: 9H8
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: BSA Free

Claudin-6 Antibody (9H8) - BSA Free Summary

• Description: Novus Biologicals Human Claudin-6 Antibody (9H8) - BSA Free (NBP3-26651) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: Recombinant Human Claudin-6 protein [UniProt P56747]
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: CLDN6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol, 0.01M PBS, PH 7.4
• Preservative: 0.03% Proclin 300
• Purity: Affinity purified

Alternate Names for Claudin-6 Antibody (9H8) - BSA Free

• claudin 6
• Claudin6
• Claudin-6
• CLDN6
• Skullin 2
• skullin

Background

The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-6 is expressed in differentiated F9 cells that resemble tight junction-bearing viceral endoderm resulting from stimulation with retinoc acid and mediated by RXRalpha and RARgamma. Claudin-6 is absent in mouse brain and lung. The human claudin-6 gene maps to chromosome 16p13.3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN6