Claudin-18 Antibody (25E3) - BSA Free

ELISA: Claudin-18 Antibody (25E3) [NBP3-26657] - The Binding Activity of Human Claudin-18.2 with Claudin-18 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Claudin-18.2 at 5 ug/mL can bind Claudin-18 Antibody, the EC50 is 2.947-8.782 ng/mL.

Flow Cytometry: Claudin-18 Antibody (25E3) [NBP3-26657] - Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

• Reactivity: Hu
• Applications: ELISA, Flow
• Clone: 25E3
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: BSA Free

Claudin-18 Antibody (25E3) - BSA Free Summary

• Description: Novus Biologicals Human Claudin-18 Antibody (25E3) - BSA Free (NBP3-26657) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: Recombinant Human Claudin-18 protein [UniProt P56856]
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: CLDN18
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol, 0.01M PBS, PH 7.4
• Preservative: 0.03% Proclin 300
• Purity: Affinity purified

Alternate Names for Claudin-18 Antibody (25E3) - BSA Free

• claudin 18
• Claudin18
• Claudin-18
• CLDN18
• SFTA5
• SFTPJ
• surfactant associated 5
• surfactant associated protein J
• surfactant, pulmonary associated protein J

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN18