Claudin-1 Antibody (CL3698) - BSA Free

Western Blot: Claudin-1 Antibody (CL3698) [NBP2-61630]-Analysis in human cell lines RT-4 and U-251MG using Anti-CLDN1 antibody. Corresponding CLDN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: CL3698
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Claudin-1 Antibody (CL3698) - BSA Free Summary

• Description: Novus Biologicals Mouse Claudin-1 Antibody (CL3698) - BSA Free (NBP2-61630) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O95832, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Control Peptide:

  Claudin-1 Recombinant Protein Antigen (NBP2-61630PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Claudin-1 Antibody (CL3698) - BSA Free

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-1 Antibody (NBP2-61630)(1)

Publication using NBP2-61630

• Watanabe‐Yasuoka Y, Watanabe R, Gotou A et al. Lactiplantibacillus plantarum OLL2712 Protects From the Intestinal Dysfunction in D‐Galactose Induced Senescent Cells Food science & nutrition 2025-09-28 [PMID: 41030836]

Bioinformatics

• Gene Symbol: CLDN1