Claudin-1 Antibody (421203) [Unconjugated]

PC‑3 human prostate cancer cell line was stained with Rat Anti-Human Claudin‑1 Monoclonal Antibody (Catalog # MAB4618, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).

(A) iPSC-derived pancreatic duct-like organoids were fixed and stained with a Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red) or with a (B) Mouse Anti-Human KRT19 Monoclonal Antibody (Catalog # MAB35061; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red), followed by secondary antibody staining with the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009) and NorthernLights 557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid. Images were taken using a Leica THUNDER Imaging System (scale bar: 20 mm).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 421203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Claudin-1 Antibody (421203) [Unconjugated] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with human Claudin-1
  Met1-Val211
  Accession # O95832
• Specificity: Stains human Claudin-1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLDN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-1 Antibody (421203) [Unconjugated]

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-1 Antibody (MAB4618)(5)

Publications using MAB4618

• SP Hong, TE Chan, Y Lombardo, G Corleone, N Rotmensz, S Bravaccini, A Rocca, G Pruneri, KR McEwen, RC Coombes, I Barozzi, L Magnani Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy Nat Commun, 2019-09-02;10(1):3840. 2019-09-02 [PMID: 31477698] (Flow Cytometry, Human)
• SS Han, SH Yang, HA Jo, YJ Oh, M Park, JY Kim, H Lee, JP Lee, SH Lee, KW Joo, CS Lim, YS Kim, DK Kim BAFF and APRIL expression as an autoimmune signature of membranous nephropathy Oncotarget, 2017-12-14;9(3):3292-3302. 2017-12-14 [PMID: 29423047] (Flow Cytometry, Human)
• Peiqi Yin, Ye Li, Leiliang Zhang Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry Journal of Virology 9/15/2017 [PMID: 28679754]
• Mailly L, Xiao F, Lupberger J, Wilson G, Aubert P, Duong F, Calabrese D, Leboeuf C, Fofana I, Thumann C, Bandiera S, Lutgehetmann M, Volz T, Davis C, Harris H, Mee C, Girardi E, Chane-Woon-Ming B, Ericsson M, Fletcher N, Bartenschlager R, Pessaux P, Vercauteren K, Meuleman P, Villa P, Kaderali L, Pfeffer S, Heim M, Neunlist M, Zeisel M, Dandri M, McKeating J, Robinet E, Baumert T Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody. Nat Biotechnol, 2015-03-23;33(5):549-54. 2015-03-23 [PMID: 25798937] (IHC, Neutralization, Human, Mouse)
• Farquhar MJ, Hu K, Harris HJ Hepatitis C virus induces CD81 and claudin-1 endocytosis. J. Virol., 2012-02-08;86(8):4305-16. 2012-02-08 [PMID: 22318146] (ICC, Human)

Review for Claudin-1 Antibody (MAB4618) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23704991
• Species: Other

Bioinformatics

• Gene Symbol: CLDN1
• Uniprot:
  • O95832()