Claudin-1 Antibody (421203) [Allophycocyanin]

PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4618A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 421203
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Claudin-1 Antibody (421203) [Allophycocyanin] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with human Claudin-1
  Met1-Val211
  Accession # O95832
• Specificity: Stains human Claudin-1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLDN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-1 Antibody (421203) [Allophycocyanin]

• claudin 1
• Claudin1
• Claudin-1
• CLD1
• CLDN1
• ILVASCSenescence-associated epithelial membrane protein
• SEMP1
• SEMP1senescence-associated epithelial membrane protein 1

Background

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-1 Antibody (FAB4618A)(1)

Publication using FAB4618A

• Giugliano S, Petroff M, Warren B, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko M, Gale M, Golden-Mason L, Winn V, Rosen H Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission. J Immunol, 2015-09-04;195(8):3737-47. 2015-09-04 [PMID: 26342030] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLDN1
• Uniprot:
  • O95832()