Home » CL-P1/COLEC12 » Colec12 Research Products » CL-P1/COLEC12 Antibody

CL-P1/COLEC12 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Colec12 Antibody [NBP1-59015] - Human Liver cell lysate, concentration 0.2-1 ug/ml.
Western Blot: Colec12 Antibody [NBP1-59015] - Human Liver cell lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Eq, GP, RbSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

CL-P1/COLEC12 Antibody Summary

Immunogen
Synthetic peptides corresponding to COLEC12(collectin sub-family member 12) The peptide sequence was selected from the middle region of COLEC12. Peptide sequence AGERGPIGPAGPPGERGGKGSKGSQGPKGSRGSPGKPGPQGPSGDPGPPG.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COLEC12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against COLEC12 and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for CL-P1/COLEC12 Antibody

  • CL-P1
  • CLP1hCL-P1
  • COLEC12
  • collectin placenta 1
  • Collectin placenta protein 1
  • collectin sub-family member 12
  • collectin-12
  • NSR2
  • Nurse cell scavenger receptor 2
  • SCARA4
  • SCARA4NSR2
  • Scavenger receptor class A member 4
  • scavenger receptor class A, member 4
  • SRCL Type I
  • SRCLScavenger receptor with C-type lectin

Background

COLEC12 is a member of the C-lectin family,¡¡proteins that possess collagen-like sequences and carbohydrate recognition domains. This protein is a scavenger receptor, a cell surface glycoprotein that can bind to carbohydrate antigens on microorganisms facilitating their recognition and removal. In addition, these receptors can recognize oxidized phospholipids so they may also participate in removing oxidatively damaged or apoptotic cells.This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. This protein is a scavenger receptor, a cell surface glycoprotein that can bind to carbohydrate antigens on microorganisms facilitating their recognition and removal. In addition, these receptors can recognize oxidized phospholipids so they may also participate in removing oxidatively damaged or apoptotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CDC14 Antibody
(472508) [Unconjugate
MAB4457
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4‑II‑E‑C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB
PCF11 Antibody
NBP2-12787
Western Blot: PCF11 Antibody [NBP2-12787] - Detection of human PCF11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PCF11 antibody NBP2-12787 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PCF11 was also immunoprecipitated by rabbit anti-PCF11 antibody NBP2-12786, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Species: Hu
Applications: WB, IP
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(10 Reviews)
(39 Publications)
Species: Hu
Applications: WB, IHC, IP
RPGR Antibody
NBP1-84412
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human soft tissues shows no positivity in adipocytes.
(2 Publications)
Species: Hu
Applications: IHC, IHC-P
CRX/CORD2 Antibody
(4G11)
H00001406-M02
Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M02), clone 4G11.Lane 1: CRX transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Retinal development is recapitulated in differentiating stem cells. Immunostaining of day 49 cultures shows remnants of retinal organization. CRX+ photoreceptor progenitors in green and mCherry+ RGCs in magenta. CRX+ cells - white arrows - appear to segregate from mCherry+ axons, suggesting division between the outer nuclear layer and the nerve fiber layer. Scale bar?=?500?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep16595), licensed under a CC-BY licence.
Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - CRX monoclonal antibody (M02), clone 4G11 Analysis of CRX expression in IMR-32.
(19 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF
CDC2/CDK1 Antibody
NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
DC-SIGN/CD209
Antibody (120507)
MAB161
Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
(1 Review)
(34 Publications)
Species: Hu
Applications: WB, Flow, IHC, AdBlk, CyTOF-ready, ICC
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
CEACAM5/CD66e
Antibody (CEA31)
NBP2-33012
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human colon stained with CEA MAb (CEA31)
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) [NBP2-33012] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).
Species: Hu, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(1 Publication)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
LRH-1/NR5A2
Antibody
NBP2-27196
Western Blot: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2 in HEPG2 lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse NIH3T3 and 4) RAW lysate probed with 3 ug/ml of NR5A2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: LRH-1/NR5A2 Antibody [NBP2-27196] - Analysis of NR5A2/LRH1 in human pancreas tissue using NR5A2/LRH1 antibody at 5 ug/ml.
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, ChHa, Eq, GP, Op, Pm, Rb, RM, Ze
Applications: WB, IHC, IHC-P
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(1 Publication)
Species: Hu
SR-BI Antibody
NB400-104
Simple Western: SR-BI Antibody [NB400-104] - Image shows a specific band for SR-BI in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: SR-BI Antibody [NB400-104] - Analysis using the HRP-conjugate of NB400-104. Detection of SR-BI in 25 ug of mouse liver lysate, using NB400-104 (lotJ) at a 1:1000 dilution, shows distinct band at 82 kDa.
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins. Photo courtesy of product review by verified customer.
(4 Reviews)
(186 Publications)
Species: Hu, Mu, Rt, Po, ChHa, SyHa, Ha, Md, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MARCO Antibody
NBP2-39004
Immunocytochemistry/Immunofluorescence: MARCO Antibody [NBP2-39004] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry: MARCO Antibody [NBP2-39004] - Staining in normal human liver. Image from a verified customer review.
Immunohistochemistry-Paraffin: MARCO Antibody [NBP2-39004] - Staining of human liver shows strong positivity in Kupffer cells.
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Recombinant Human
ELL Protein
H00008178-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
LOX-1/OLR1
Antibody (331212) [Un ...
MAB1798
THP‑1 human acute monocytic leukemia cell line were treated for 72 hours with 50 ng/mL PMA then stained with Mouse Anti-Human LOX‑1/OLR1 Monoclonal Antibody (Catalog # MAB1798, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
(7 Publications)
Species: Hu
Applications: WB, Flow, Block, CyTOF-ready

Publications for CL-P1/COLEC12 Antibody (NBP1-59015) (0)

There are no publications for CL-P1/COLEC12 Antibody (NBP1-59015).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CL-P1/COLEC12 Antibody (NBP1-59015) (0)

There are no reviews for CL-P1/COLEC12 Antibody (NBP1-59015). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CL-P1/COLEC12 Antibody (NBP1-59015) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional CL-P1/COLEC12 Products

Bioinformatics Tool for CL-P1/COLEC12 Antibody (NBP1-59015)

Discover related pathways, diseases and genes to CL-P1/COLEC12 Antibody (NBP1-59015). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CL-P1/COLEC12 Antibody (NBP1-59015)

Discover more about diseases related to CL-P1/COLEC12 Antibody (NBP1-59015).
 

Pathways for CL-P1/COLEC12 Antibody (NBP1-59015)

View related products by pathway.

PTMs for CL-P1/COLEC12 Antibody (NBP1-59015)

Learn more about PTMs related to CL-P1/COLEC12 Antibody (NBP1-59015).

Blogs on CL-P1/COLEC12

There are no specific blogs for CL-P1/COLEC12, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CL-P1/COLEC12 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol COLEC12
Uniprot