CIP2A Antibody

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Immunohistochemistry: CIP2A Antibody [NB100-74663] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:250. Detection: DAB staining using ...read more
Immunohistochemistry: CIP2A Antibody [NB100-74663] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:250. Detection: DAB staining using ...read more
Immunohistochemistry: CIP2A Antibody [NB100-74663] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A used at a dilution of 1:100. Detection: Red-fluorescent goat

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CIP2A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human cancerous inhibitor of PP2A using the numbering given in entry NP_065941.1 (GeneID 57650).
Localization
Membrane; Single-pass membrane protein(Potential). Cytoplasm. Note=Slightly concentrates in the perinuclear region
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIAA1524
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Epitope exposure with citrate buffer exposure is recommended to enhance staining. Although not tested this antibody may be useful in frozen sections and Immunoflourescence/Immunocytochemistry Western Blot was reported in scientific literature.Use in Flow Cytometry reported in scientific literature (PMID 20447748)
Positive Control
CIP2A Lysate (NBL1-12266)
Publications
Read Publications using
NB100-74663 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • 5 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20447748)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CIP2A Antibody

  • Cancerous inhibitor of PP2A
  • CIP2A
  • FLJ12850
  • KIAA1524
  • MGC163436
  • p90 autoantigen
  • p90
  • protein CIP2A

Background

CIP2A (cancerous inhibitor of PP2A) was originally identified as p90, a cytoplasmic autoantigen found in the serum from a cancer patient. It was later described as a protein that interacted with the c-myc oncogene and functioned as an inhibitor of protein phosphatase 2A (PP2A) activity. Inhibition of PP2A phosphatase activity by CIP2A prevents c-myc phosphorylation and counteracts c-myc proteolytic degradation, thereby stabilizing c-myc and promoting cell growth and transformation. CIP2A is found to be overexpressed in some human malignancies and is thus classified as an oncogene that may be an important contributor to tumor formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIP2A Antibody (NB100-74663)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, IHC, IHC-P, WB.


Filter By Application
FLOW
(1)
IHC
(2)
IHC-P
(5)
WB
(2)
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Human
(7)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-74663 Applications Species
Li Y, Cai X, Chen B et al. Overexpression of epithelial cell transforming 2 protein in colorectal carcinoma predicts a poor prognosis Experimental and Therapeutic Medicine 2017 Sep 19 [PMID: 29109759] (Human) Human
Chen KF, Yen CC, Lin JK et al. Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an independent prognostic marker in wild-type KRAS metastatic colorectal cancer after colorectal liver metastasectomy BMC Cancer 2015 Apr 21 [PMID: 25896895] (WB, IHC-P, Human) WB, IHC-P Human
Florenes VA, Emilsen E, Dong HP et al. Cellular localization of CIP2A determines its prognostic impact in superficial spreading and nodular melanoma Cancer Med. 2015 Feb 07 [PMID: 25663244] (IHC-P, Human) IHC-P Human
Wang Hsei-Wei, Yang Shung-Haur, Huang Guan-Da et al. Temsirolimus enhances the efficacy of cetuximab in colon cancer through a CIP2A-dependent mechanism. J Cancer Res Clin Oncol. 2014 Feb 04 [PMID: 24493623]
He H, Wu G, Li W et al. CIP2A is highly expressed in hepatocellular carcinoma and predicts poor prognosis. Diagn Mol Pathol. 2012 Sep [PMID: 22847158] (IHC-P, Human) IHC-P Human
Teng HW, Yang SH, Lin JK, Chen WS, Lin TC, Jiang JK, Yen CC, Li AF, Chen PC, Lan YT, Lin CC, Hsu YN, Wang HW, Chen KF. CIP2A Is a Predictor of Poor Prognosis in Colon Cancer. J Gastrointest Surg;16(5):1037-47. 2012 May. [PMID: 22328001] (IHC, WB, Human) IHC, WB Human
Bockelman C, Lassus H, Hemmes A et al. Prognostic role of CIP2A expression in serous ovarian cancer. British Journal of Cancer. 2011 Sep 6. [PMID: 21897396]
Vaarala MH, Vaisanen MR, Ristimaki A. CIP2A expression is increased in prostate cancer J Exp Clin Cancer Res 2010 Oct 21 [PMID: 20964854] (IHC, Human) IHC Human
Kerosuo L, Fox H, Perala N et al. CIP2A increases self-renewal and is linked to Myc in neural progenitor cells. Differentiation. 2010 Jul [PMID: 20447748] (IHC-P, FLOW, Mouse) IHC-P, FLOW Mouse
Bockelman C. Role of CIP2A in carcinogenesis Thesis University of Helsinki, Haartman Institute, Patologia URN:ISBN:978-952-10-7579-7. 2012 Feb 10 (IHC-P, Mouse, Human) IHC-P Mouse, Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CIP2A Antibody (NB100-74663) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CIP2A.

CIP2A - A lean, mean, oncoprotein machine
The CIP2A protein was originally identified as p90, a cytoplasmic auto-antigen from the serum of a cancer patient. It was later found to inhibit protein phosphatase 2A (PP2A) activity as well as interact with c-myc. CIP2A's inhibitory activity blocks...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

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Bioinformatics

Gene Symbol KIAA1524
Entrez
Uniprot