Cingulin Antibody - BSA Free

Western Blot: Cingulin Antibody [NBP2-15904] - Sample (30 ug of whole cell lysate) A: Juakat 7. 5% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-15904] - Cytoplasm on Ovarian carcinoma; antibody dilution: 1:500.

Immunocytochemistry/ Immunofluorescence: Cingulin Antibody [NBP2-15904] - Cingulin antibody detects Cingulin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cingulin stained by Cingulin antibody (NBP2-15904) diluted at 1:500.

Western Blot: Cingulin Antibody [NBP2-15904] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cingulin antibody (NBP2-15904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cingulin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cingulin Antibody - BSA Free (NBP2-15904) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Cingulin. The exact sequence is proprietary.
• Localization: Cell junction; tight junction
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CGN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (86%), Rabbit (83%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cingulin Antibody - BSA Free

• cingulin
• DKFZp779N1112
• KIAA1319

Background

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CGN