CILP-1 Antibody

Western Blot: CILP-1 Antibody [NB100-41106] - (0.03ug/ml) staining of rat spinal cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CILP-1 Antibody Summary

• Description: Novus Biologicals Goat CILP-1 Antibody (NB100-41106) is a polyclonal antibody validated for use in WB and ELISA. Anti-CILP-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NYRRTDHEDPRVK corresponding to internal region according to NP_003604.2.
• Predicted Species: Human (100%), Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CILP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in rat spinal cord lysates (calculated MW of 133 kDa band according to human NP_003604.2 and of 132 kDa band according to rat NP_001101631.1).
• Control:

  CILP-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CILP-1 Antibody

• cartilage intermediate layer protein 1 C1
• cartilage intermediate layer protein 1 C2
• cartilage intermediate layer protein 1
• cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• Cartilage intermediate-layer protein
• CILP
• CILP1
• CILP-1
• HsT18872

Background

CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CILP-1 Antibody (NB100-41106)(1)

Publication using NB100-41106

• Seki S, Kawaguchi Y, Chiba K et al. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat Genet 2005-06-01 [PMID: 15864306]

Bioinformatics

• Gene Symbol: CILP
• Entrez:
  • 8483(Human)
  • 214425(Mouse)
  • 315761(Rat)
• Uniprot:
  • NP_003604.2(Human)