CILP-1 Antibody

Images

 
Western Blot: CILP-1 Antibody [NB100-41106] - (0.03ug/ml) staining of rat spinal cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CILP-1 Antibody [NB100-41106] - CILP using NB100-41106 in Rat Spinal Cord lysate (35ug protein in RIPA buffer) at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CILP-1 Antibody Summary

Immunogen
Peptide with sequence C-NYRRTDHEDPRVK corresponding to internal region according to NP_003604.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CILP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 150 kDa band observed in rat spinal cord lysates (calculated MW of 133 kDa band according to human NP_003604.2 and of 132 kDa band according to rat NP_001101631.1).
Positive Control
CILP-1 Lysate (NBL1-09208)
Publications
Read Publication using NB100-41106.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CILP-1 Antibody

  • cartilage intermediate layer protein 1 C1
  • cartilage intermediate layer protein 1 C2
  • cartilage intermediate layer protein 1
  • cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
  • Cartilage intermediate-layer protein
  • CILP
  • CILP1
  • CILP-1
  • HsT18872

Background

CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CILP-1 Antibody (NB100-41106)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CILP
Entrez
Uniprot