CILP-1 Overexpression Lysate

Images

 
Western Blot: CILP Overexpression Lysate (Adult Normal) [NBL1-09208] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CILP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CILP-1 Overexpression Lysate Summary

Description
Transient overexpression lysate of cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP) The lysate was created in HEK293T cells, using Plasmid ID RC207801 and based on accession number NM_003613. The protein contains a C-MYC/DDK Tag.
Gene
CILP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CILP-1 Overexpression Lysate

  • cartilage intermediate layer protein 1 C1
  • cartilage intermediate layer protein 1 C2
  • cartilage intermediate layer protein 1
  • cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
  • Cartilage intermediate-layer protein
  • CILP
  • CILP1
  • CILP-1
  • HsT18872

Background

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for 2 different proteins, namely CILP and a homolog of NTPPHase, however later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. One isoform of the protein, CILP-1, functions as an IGF-1 antagonist. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CILP-1 Lysate (NBL1-09208) (0)

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-09208

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Bioinformatics

Gene Symbol CILP