CHURC1 Antibody - BSA Free

Western Blot: CHURC1 Antibody [NBP1-94125] - Analysis in control (vector only transfected HEK293T lysate) and CHURC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: CHURC1 Antibody [NBP1-94125] - Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.

Immunohistochemistry-Paraffin: CHURC1 Antibody [NBP1-94125] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Analysis in control (vector only transfected HEK293T lysate) and CHURC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Staining of human cell line PC-3 shows localization to cytosol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CHURC1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GDCVEKEYPNRGNTCLENGSFLLNFTGCAVCSKRDFMLITNKSLKEEDGEEIVTYDHLCKNCHHVIARHEYTFSIMDEFQEYTMLCLLCGKAEDTISILPDDP
• Predicted Species: Mouse (96%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHURC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CHURC1 Protein (NBP1-94125PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CHURC1 Antibody - BSA Free

• C14orf52
• chch
• churchill domain containing 1
• FLJ33064
• FLJ51978
• FLJ78804
• My015
• protein Churchill

Background

Transcriptional activator that mediates FGF signaling during neural development. Plays a role in theregulation of cell movement . Does not bind DNA by itself

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHURC1
• Entrez:
  • 91612(Human)
• Uniprot:
  • Q8WUH1(Human)