CHTF18 Antibody - BSA Free

Western Blot: CHTF18 Antibody [NBP2-58935] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: CHTF18 Antibody [NBP2-58935] - Staining of human cell line MCF7 shows localization to nucleoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CHTF18 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CHTF18 Antibody - BSA Free (NBP2-58935) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GIGEKGVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHTF18
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CHTF18 Recombinant Protein Antigen (NBP2-58935PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CHTF18 Antibody - BSA Free

• C16orf41
• C321D2.2
• C321D2.3 (novel protein)
• C321D2.3
• C321D2.4 (novel protein)
• CHL12C321D2.4
• chromosome 16 open reading frame 41
• chromosome transmission fidelity protein 18 homolog
• Ctf18
• CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
• EC 3.6.1
• hCTF18
• homolog of yeast CHL12
• RUVBL
• some homology with holliday junction DNA helicase RUVB like

Background

CTF18 (Chromosome Transmission Fidelity protein 18 homolog) is part of the CTF18-RFC (Replication Factor C) complex composed of CTF18, CTF8, DCC1, and four RFC subunits. This complex has been shown to be important for the establishment of sister chromatid cohesion and functions as a proliferating cell nuclear antigen (PCNA) loader for DNA synthesis. CTF18 is also known as hCTF18, CHTF18, CHL12, and RUVBL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHTF18