CHSS2 Antibody

Western Blot: CHSS2 Antibody [NBP1-88087] - Analysis in control (vector only transfected HEK293T lysate) and CHPF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: CHSS2 Antibody [NBP1-88087] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, centrosome & vesicles.

Immunohistochemistry-Paraffin: CHSS2 Antibody [NBP1-88087] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CHSS2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDRAAAWPVGIPAPS
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHPF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CHSS2 Protein (NBP1-88087PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%). Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CHSS2 Antibody

• Chondroitin glucuronyltransferase 2
• chondroitin polymerizing factor
• chondroitin sulfate synthase 2
• Chondroitin-polymerizing factor
• ChPF
• CHSY2
• CSS2FLJ22678
• EC 2.4.1.175
• EC 2.4.1.226
• Glucuronosyl-N-acetylgalactosaminyl-proteoglycan4-beta-N-acetylgalactosaminyltransferase II
• N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II
• N-acetylgalactosaminyltransferase 2

Background

CHSS2 has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfersglucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end ofthe elongating chondroitin polymer

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHSS2 Antibody (NBP1-88087)(1)

Publication using NBP1-88087

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: CHPF