Recombinant Human CHST10 Protein, CF


There are currently no images for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human CHST10 Protein, CF Summary

Details of Functionality
Measured by its ability to transfer sulfate from PAPS to phenolpthalein glucuronic acid. The specific activity is >350 pmol/min/μg, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human Carbohydrate Sulfotransferase 10/CHST10 protein
Pro32-Asn356, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Enzyme Activity
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
55-75 kDa, reducing conditions
Read Publication using
6140-ST in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Assay Procedure
  • Assay Buffer (provided in kit): 50 mM Tris, 15 mM MgCl2, pH 7.5
  • Recombinant Human Carbohydrate Sulfotransferase 10/CHST10 (rhCHST10) (Catalog # 6140-ST)
  • 3'-Phosphoadenosine-5'-phosphosulfate/PAPS (Catalog # ES019)
  • Phenolphthalein glucuronic acid (PGA)  (Sigma, Catalog # P0501), 0.1 M stock in deionized water
  • Universal Sulfotransferase Activity Kit (Catalog # EA003)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute 1 mM Phosphate Standard provided by the Universal Sulfotransferase Kit by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 µM stock.
  2. Prepare standard curve by performing six one‑half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5 nmol per well.
  3. Prepare reaction mixture containing 0.4 mM PAPS, 1 mM PGA, and 20 μg/mL Coupling Phosphatase 3 in Assay Buffer.
  4. Dilute rhCHST10 to 4 µg/mL in Assay Buffer.
  5. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
  6. Load 25 µL of the 4 µg/mL rhCHST10 into the plate. Include a Control containing 25 µL of Assay Buffer.
  7. Add 25 µL of reaction mixture to the wells, excluding the standard curve.
  8. Cover the plate with a plate sealer and incubate at 37 °C for 20 minutes.
  9. Add 30 µL of the Malachite Green Reagent A to all wells. Mix briefly.
  10. Add 100 µL of deionized water to all wells. Mix briefly.
  11. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  12. Read plate at 620 nm (absorbance) in endpoint mode.
  13. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

Per Reaction:
  • rhCHST10: 0.1 μg
  • Coupling Phosphatase 3: 0.5 μg
  • PAPS: 0.2 mM
  • PGA: 0.5 mM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CHST10 Protein, CF

  • Carbohydrate Sulfotransferase 10
  • CHST10
  • EC 2.8.2
  • HNK-1 sulfotransferase
  • HNK1ST
  • HNK-1STEC 2.8.2.-
  • huHNK-1ST
  • MGC17148


CHST10 is the only known sulfotransferase that catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides (1, 2). CHST10 is a key enzymes for the synthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules (3). HNK-1 is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. In adults, CHST10 is highly expressed in brain, testis and ovary. Recently, it is reported that CHST10 functions as a suppressor of invasiveness of human melanoma cells (4). Like many other carbohydrate specific sulfotransferases, CHST10 is a single-pass type II membrane protein and is localized in Golgi apparatus membrane. The recombinant human CHST10 only contains the luminal enzymatic domain and the enzymatic activity is measured using a phosphatase-coupled assay (5).
  1. Ong, E. et al. (1999) J. Biol. Chem. 274:25608.
  2. Ong, E. et al. (1998) J. Biol. Chem. 273:5190.
  3. Kizuka, Y. et al. (2006) J. Biol. Chem. 281:13644.
  4. Zhao, X. et al. (2009) Cancer Res. 69:5218.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: IHC, WB
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Mu
Applications: WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
Species: Fe, Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Mu, Rt
Applications: IHC, WB
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: Enzyme Activity

Publications for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST)(1)

We have publications tested in 2 confirmed species: Bovine, Porcine.

We have publications tested in 1 application: Enzyme Assay.

Filter By Application
Enzyme Assay
All Applications
Filter By Species
All Species

Reviews for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST) (0)

There are no reviews for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Carbohydrate Sulfotransferase 10/CHST10 Products

Bioinformatics Tool for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST)

Discover related pathways, diseases and genes to Carbohydrate Sulfotransferase 10/CHST10 (6140-ST). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST)

Discover more about diseases related to Carbohydrate Sulfotransferase 10/CHST10 (6140-ST).

Pathways for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST)

View related products by pathway.

PTMs for Carbohydrate Sulfotransferase 10/CHST10 (6140-ST)

Learn more about PTMs related to Carbohydrate Sulfotransferase 10/CHST10 (6140-ST).

Blogs on Carbohydrate Sulfotransferase 10/CHST10

There are no specific blogs for Carbohydrate Sulfotransferase 10/CHST10, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human CHST10 Protein, CF and receive a gift card or discount.


Gene Symbol CHST10