Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hFSH-132) - (Alpha 4 epitope) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: INN-hFSH-132
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (INN-hFSH-132) - (Alpha 4 epitope) - BSA Free Summary

• Immunogen: Human Follicle Stimulating Hormone (FSH)
• Specificity: A high affinity antibody recognising the alpha 4 epitope of the alpha-subunit of Human Chorionic Gonadotrophin (hCG), hLH, hFSH, hTSH and free alpha-subunit. The recognised epitope comprises amino-acids 15-22 of the human sequence. This antibody will not cross-react with glycoprotein hormones of various animal species.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Control:

  Chorionic Gonadotropin alpha Chain (hCG alpha) Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Array

• alpha HCG
• CGA glycoprotein hormones, alpha polypeptide
• CGA
• CG-ALPHA
• Chorionic Gonadotropin alpha Chain
• FSHA
• GPHa
• GPHa1
• HCG
• LHA
• TSHA

Background

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chorionic Gonadotropin alpha Chain (alpha HCG) Antibody (NB100-62251)(1)

Publication using NB100-62251

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: CGA
• Entrez:
  • 1081(Human)
• Uniprot:
  • P01215()
  • P01215()