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Choline Kinase beta Antibody

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Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Choline Kinase beta Antibody Summary

Immunogen
Peptide with sequence C-KGETLSQEEQRKLEED corresponding to internal region according to NP_005189.2.
Epitope
C terminus
Specificity
This antibody is expected to recognize isoform a (NP_005189.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHKB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3 kDa band according to NP_005189.2. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-28466.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Choline Kinase beta Antibody

  • CHETK
  • CHETKcholine/ethanolamine kinase beta
  • CHKL
  • CHKLcholine kinase-like
  • ChoKB
  • Choline Kinase beta
  • choline kinase betaEK
  • Choline kinase-like protein
  • CK
  • CKB
  • CKEKB
  • CKEKBEKBcholine/ethanolamine kinase
  • EC 2.7.1.32
  • EC 2.7.1.82
  • EK
  • EKB
  • Ethanolamine Kinase beta
  • Ethanolamine kinase
  • MDCMC

Background

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Choline Kinase
beta Antibody
NBP1-28466
Choline Kinase beta Antibody
Species: Hu, Ca
Applications: PEP-ELISA

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Bioinformatics

Gene Symbol CHKB
Entrez
Uniprot