Choline Acetyltransferase/ChAT Antibody

Images

 
Western Blot: Choline Acetyltransferase Antibody [NB300-910] - Analysis of Choline Acetyltransferase in Human Placenta lysate using this antibody at 0.03 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Choline Acetyltransferase/ChAT Antibody Summary

Immunogen
Peptide with sequence CKEKATRPSQGHQP corresponding to C-Terminus according to NP_066264.3, NP_065574.3, NP_001136405.1.
Marker
Cholinergic Neuronal Marker
Specificity
This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 70 kDa band observed in human placenta lysates (calculated MW of 70.5 kDa band according to NP_065574 and NP_066264).
Publications
Read Publication using NB300-910.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22449
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, GS
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NB100-91348
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-45897
Species: Hu
Applications: WB, IHC
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
MAB367
Species: Hu, Ca
Applications: WB, IHC, ICC
AF2086
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP1-05163
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
AF9024
Species: Mu, Rt
Applications: WB, IHC
NBP2-50048
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF

Publications for Choline Acetyltransferase/ChAT Antibody (NB300-910)(1)

Reviews for Choline Acetyltransferase/ChAT Antibody (NB300-910) (0)

There are no reviews for Choline Acetyltransferase/ChAT Antibody (NB300-910). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Choline Acetyltransferase/ChAT Antibody (NB300-910) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Choline Acetyltransferase/ChAT Products

Bioinformatics Tool for Choline Acetyltransferase/ChAT Antibody (NB300-910)

Discover related pathways, diseases and genes to Choline Acetyltransferase/ChAT Antibody (NB300-910). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Choline Acetyltransferase/ChAT Antibody (NB300-910)

Discover more about diseases related to Choline Acetyltransferase/ChAT Antibody (NB300-910).
 

Pathways for Choline Acetyltransferase/ChAT Antibody (NB300-910)

View related products by pathway.

PTMs for Choline Acetyltransferase/ChAT Antibody (NB300-910)

Learn more about PTMs related to Choline Acetyltransferase/ChAT Antibody (NB300-910).

Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Choline Acetyltransferase/ChAT Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CHAT
Entrez
Uniprot