Chk2 Antibody (4B7)

Images

 
Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7. Analysis of CHEK2 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Chk2 Antibody (4B7) [H00011200-M01] - Analysis of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: Chk2 Antibody (4B7) [H00011200-M01] - CHEK2 monoclonal antibody (M01), clone 4B7 Analysis of CHEK2 expression in Hela S3 NE.
Sandwich ELISA: Chk2 Antibody (4B7) [H00011200-M01] - Detection limit for recombinant GST tagged CHEK2 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
4B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Chk2 Antibody (4B7) Summary

Immunogen
CHEK2 (AAH04207, 1 a.a. ~ 544 a.a) full length recombinant protein with GST tag.MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRK
Specificity
CHEK2 - CHK2 checkpoint homolog (S. pombe)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHEK2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using
H00011200-M01 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Chk2 Antibody (4B7)

  • CDS1
  • CHEK2
  • CHK2 checkpoint homolog (S. pombe)
  • Chk2
  • EC 2.7.11
  • EC 2.7.11.1
  • HuCds1
  • LFS2
  • PP1425
  • Rad53
  • S.pombe) homolog

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Chk2 Antibody (H00011200-M01)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Filter By Species
Human
(2)
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Bioinformatics

Gene Symbol CHEK2
Entrez
OMIM
Uniprot