Chk1 [p Ser317] Antibody [PerCP]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: PerCP

Chk1 [p Ser317] Antibody [PerCP] Summary

• Immunogen: Phosphopeptide containing human Chk1 S317 site
• Modification: p Ser317
• Specificity: Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHEK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Chk1 [p Ser317] Antibody [PerCP]

• Checkpoint, S. pombe, homolog of, 1
• CHEK1
• CHK1 (checkpoint, S.pombe) homolog
• CHK1 checkpoint homolog (S. pombe)
• Chk1
• CHK1serine/threonine-protein kinase Chk1
• EC 2.7.11
• EC 2.7.11.1

Background

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHEK1